East Port zve na seminář/workshop:
Guidance to the sustainable bacterial endotoxin testing, practical introduction to the recombinant Factor C assay and Lonza solution for Pyrogen Testing

Seminář/Workshop
April 19th

Louvre Café Gallery Národní 22, 110 00 Prague 1

Guidance to the sustainable bacterial endotoxin testing, practical introduction to the recombinant Factor C assay and Lonza solution for Pyrogen Testing

East Port would like to invite you to a half-day seminar/workshop dedicated to bacterial endotoxins and pyrogens detection under the guidance of Lonza specialists, Dr. Ingo Ciolkowski and Jessica Marquardt. We will start with a summary of the importance of endotoxin testing and the types of tests, while focusing on the use of recombinant factor C assay. You will then have the opportunity to see a hands-on demonstration of PyroGeneTM Recombinant Factor C Endpoint Fluorescent Assay, which is part of the European Pharmacopoeia And is being implemented in the Czech Pharmacopoeia. After a refreshment break, we will introduce MAT test for the detection of pyrogens and at the end we will evaluate the results of the test with PyroGene assay.

Place of the event

19.4.2023

CAFÉ LOUVRE GALLERY
NÁRODNÍ 22, 110 00 PRAGUE 1

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Schedule of the event

9:30 – 11:00

Introduction Lonza - QC Testing
Traditional Endotoxin Assays (Gel Clot to Kinetic LAL)
PyroGene Assay & History
LAL Mechanism - Reaction Cascades & Glucans
Sustainability & Benefits of rFC

11:00 – 11:15 Coffee Break
11:15 – 12:15

Endotoxin Detection Software WinKQCL
Demo of Pyrogene
Preparing standards curve mock run for gain setting
Sample preparation (water)
Running the assay

12:15 – 13:00 Lunch break
13:00 – 14:45

Tips for implementing / validating the rFC
Current regulatory topics
Analysing and discussing the result
Introduction MAT

14:45 – 15:00 Closing remarks & Wrap up

 

For whom the event is intended

The whole event is intended for all those who are already actively testing or would like to start testing for endotoxins, pyrogens or MAT tests. The seminar/workshop will be held in English and registration is required for participation. The whole event is free of charge for all participants.