Cell Health Assays to Interrogate 3D Cell Culture Models

There is a broad spectrum of 3D cell culture models being used to achieve improved physiological relevance compared to cells grown as monolayers on plastic. Important features to consider when assaying 3D culture models are the physical size and distance for assay reagent or light penetration. Most off-the-shelf assays were designed using cells grown as monolayers or in suspension and have not been adequately validated for use with 3D culture models. We will describe a group of cell health assays (including “real-time” approaches) validated for use with 3D culture models. We will describe how the assay chemistries work, the design of appropriate controls for assaying 3D culture models, and how to combine assays to multiplex measurement of more than one marker.

Registrace zde.