Seminar Bioluminescent Reporter Assay – Powerful Tool to Study Cell Biology IMG Prague

For the Czech version, CLICK HERE.

We invite you to our next seminar which will focus on bioluminescent reporter technologies. Bioluminescent reporter assays are a powerful tool for studying gene expression, cell signaling, and protein biology. In the first part of the seminar, we will introduce the versatile NanoLuc luciferase and the NanoBiT platform. You will learn how you can improve your gene expression studies with the NanoLuc luciferase in single and dual-reporter assays and we will also show you how you can use the split NanoBiT version of the luciferase for studying different aspects of protein biology. Our invited speakers dr. David Sedlák and Professor Petr Pávek will then present their applications of bioluminescent reporters in high-throughput screening in the search for specific insecticides and the analysis of miRNA binding and nuclear receptor activation. In the end, we will share news from Promega and our other suppliers that can be combined with bioluminescent reporter technologies and provide additional information from your experiments.

Speakers

Dr. Erik Bonke (Promega)

Dr. David Sedlák (ÚMG)

Prof. Petr Pávek (FaF UK)

Dr. Vojtěch Ledvina (East Port)

Program

Presentations of our guests

Harnessing Luciferase Reporter Assays for Eco-Friendly Insecticides: Targeting Pests with Precision – dr. David Sedlák

Our work focuses on identifying highly selective insecticides that target the juvenile hormone receptor which is unique to insects. This receptor controls insect development and targeting its physiological function opens new ways to the development of eco-friendly insecticides with potentially low impact on the ecosystem and human health. He will show you how combining expertise in insect endocrinology, high-throughput screening using dual luciferase assays, chemical and structural biology, and medicinal chemistry can lead to juvenoids that target species from evolutionarily distant taxa.

Luciferase reporter assays for the analysis of transcription factor activation and miRNA effect – prof. Petr Pávek

In this talk, I will describe our application of firefly luciferase reporter assays in both transiently and stable transfected cells. I will focus on firefly luciferase and discuss their applications for nuclear receptor activation studies and the study of miRNA function in epigenetic regulation of gene expression. I will also mention assays that we use for the detection of Firefly and Renilla luciferases and the pitfalls of luciferase reporter assays.

Who is the seminar for?

The seminar is accessible to all interested parties regardless of their occupation and age and is also suitable for university students. Registration is required for all participants due to capacity reasons. The seminar will be held in English.

Does the time and date of the seminar collide with your schedule and you cannot attend? Do not worry, we can visit you with our colleague from Promega directly at your workplace and discuss your project or your application questions there. In these cases, please contact us via email: ondrej.ptacek@eastport.cz or by phone: +420 602 200 982.